Objective Explore the effect of long-term closed environment on crew’s inhibitory function and its neurophysiological mechanism. Methods Behavioral data and EEG data were collected when the subjects completed the emotional Stroop color word test. From the psychological and physiological point of view, this paper compares the Stroop effect and accuracy and the power spectrum density of EEG signals after positive, neutral and negative emotions were induced in the crew of long sailing time group and short sailing time group. Results and Conclusion In behavioral indicators, the main effect of stimulus type is significant (F(1,42)= 3.331, p=0.041), and the accuracy of the two groups under neutral stimulus is higher than that under positive and negative stimulus. In terms of EEG indexes, there are significant differences in power values induced by positive, neutral and negative emotions between the two groups in delta and beta bands. Under positive, negative and neutral emotional stimulus, there are significant differences in power values in delta, theta and beta bands. The crew who has been working and living in a closed environment for a long time have not experienced a significant decline in behavioral inhibition, but the brain electric power has changed regionally, and the difference is mainly concentrated in the crew who have been sailing for a long time (77.7%). The innovation of the article Emotional Stroop paradigm and power spectral density are combined to explore the changes of crew’s inhibitory function in long-term closed environment.